更新時(shí)間:2016-06-13
改良克氏雙糖鐵培養(yǎng)基,年中放價(jià)銷售了,低價(jià)、*、優(yōu)惠價(jià)供應(yīng),微生物培養(yǎng)基折扣價(jià)提供,BD培養(yǎng)基、Gibco培養(yǎng)基、Amresco培養(yǎng)基、OXOID培養(yǎng)基等國內(nèi)外培養(yǎng)基,我們傾情提供。
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改良克氏雙糖鐵培養(yǎng)基 | Modified KIA Iron Medium | 250g | 生化培養(yǎng)基,用于小腸結(jié)腸炎耶爾森氏菌的生化反應(yīng)篩選。 |
用途:生化培養(yǎng)基,用于小腸結(jié)腸炎耶爾森氏菌的生化反應(yīng)篩選
改良克氏雙糖鐵培養(yǎng)基配方:(g/L) | |||||||||||||||||||||||||||||
酵母粉 蛋白胨 牛肉粉 月示蛋白胨 氯化鈉 山梨醇 葡萄糖 硫酸亞鐵 硫代硫酸鈉 瓊脂 酚紅 pH值7.4 ± 0.2 | 3.0 15.0 3.0 5.0 5.0 20.0 1.0 0.2 0.3 13.0 0.024 | ||||||||||||||||||||||||||||
原理: | |||||||||||||||||||||||||||||
蛋白胨、酵母粉、牛肉粉提供氮源、維生素、礦物質(zhì);山梨醇、葡萄糖提供可發(fā)酵糖類;硫代硫酸鈉可被某些細(xì)菌還原成H2S,與鐵離子生成黑色硫化鐵;酚紅是pH指示劑,發(fā)酵糖產(chǎn)酸變黃,產(chǎn)堿變紅;氯化鈉維持均衡的滲透壓;瓊脂是培養(yǎng)基的凝固劑。 | |||||||||||||||||||||||||||||
用法: | |||||||||||||||||||||||||||||
稱取本品 65.52g,,加熱溶解于1000ml 蒸餾水中,分裝于試管, 121℃高壓滅菌 15 分鐘,備用。 | |||||||||||||||||||||||||||||
質(zhì)量控制: | |||||||||||||||||||||||||||||
在36±1℃培養(yǎng)18-24小時(shí)。
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