更新時間:2016-06-15
人結(jié)直腸癌細胞;T84,開始培養(yǎng)保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關(guān)于人身安全的實驗。
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人結(jié)直腸癌細胞;T84
形態(tài)特性 上皮樣;多角
生長特性 貼壁生長
特征特性 人結(jié)直腸癌細胞;T84T84細胞來源于一位72歲的男性結(jié)直腸癌患者的肺部轉(zhuǎn)移灶;皮下接種至BALB/c裸鼠并連續(xù)傳代23次后建立該細胞系。在裸鼠身上傳代的過程中,該腫瘤仍保持原始的結(jié)直腸癌的組織學特性。T84細胞系生長至匯合狀態(tài)時形成單層細胞,相鄰細胞間有緊密連接和細胞橋粒;該細胞有多種肽類激素和神經(jīng)遞質(zhì)的受體,維持電解質(zhì)的定向運輸;角蛋白陽性。
培養(yǎng)條件 DMEM/F12(1:1): Ham's F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS
傳代方法 1:2~1:4傳代;每周2次。
傳代情況 C5
凍存條件 基礎培養(yǎng)基+5%DMSO+20%FBS
公司細胞廣泛用于國內(nèi)院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
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纖維介素蛋白(FGL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Fibrinogen Like Protein 2 (FGL2) X16159 48T/96T Rattus norvegicus (Rat)
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原肌球蛋白2β(TPM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Tropomyosin 2 Beta (TPM2) X16163 48T/96T Rattus norvegicus (Rat)
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有機溶質(zhì)載體伴侶1(OSCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Organic Solute Carrier Partner 1 (OSCP1) X16165 48T/96T Homo sapiens (Human)
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RAS癌基因家族成員RAB5A(RAB5A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for RAB5A, Member RAS Oncogene Family (RAB5A) X16167 48T/96T Homo sapiens (Human)
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角蛋白33A(KRT33A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Keratin 33A (KRT33A) X16171 48T/96T Homo sapiens (Human)
角蛋白40(KRT40)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Keratin 40 (KRT40) X16172 48T/96T Homo sapiens (Human)
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神經(jīng)肽Y受體Y5(NPY5R)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Neuropeptide Y Receptor Y5 (NPY5R) X16177 48T/96T Homo sapiens (Human)
神經(jīng)肽Y受體Y1(NPY1R)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Neuropeptide Y Receptor Y1 (NPY1R) X16178 48T/96T Homo sapiens (Human)
神經(jīng)肽Y受體Y2(NPY2R)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Neuropeptide Y Receptor Y2 (NPY2R) X16179 48T/96T Homo sapiens (Human)
淋巴毒素β受體(LTβR)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Lymphotoxin Beta Receptor (LTbR) X16180 48T/96T Homo sapiens (Human)
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中間α-球蛋白抑制因子H5(ITIH5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Inter Alpha-Globulin Inhibitor H5 (ITIH5) X16183 48T/96T Mus musculus (Mouse)
Ⅹ組磷脂酶A2(PLA2G10)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Phospholipase A2, Group X (PLA2G10) X16184 48T/96T Homo sapiens (Human)
剪接因子3B亞基3(SF3B3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Splicing Factor 3B Subunit 3 (SF3B3) X16185 48T/96T Mus musculus (Mouse)
整合素α6(ITGα6)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Integrin Alpha 6 (ITGa6) X16186 48T/96T Rattus norvegicus (Rat)
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凝血因子ⅩⅢA1肽(F13A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Coagulation Factor XIII A1 Polypeptide (F13A1) X16188 48T/96T Rattus norvegicus (Rat)
ⅡA組磷脂酶A2(PLA2G2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Phospholipase A2, Group IIA (PLA2G2A) X16189 48T/96T Rattus norvegicus (Rat)
分泌型卷曲相關(guān)蛋白4(SFRP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Secreted Frizzled Related Protein 4 (SFRP4) X16190 48T/96T Rattus norvegicus (Rat)
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色胺酸羥化酶2(TPH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Tryptophan Hydroxylase 2 (TPH2) X16193 48T/96T Rattus norvegicus (Rat)
內(nèi)向整流型鉀離子通道亞家族J成員5(KCNJ5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) X16194 48T/96T Rattus norvegicus (Rat)
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蛋白L-異天冬氨酸-O-甲基轉(zhuǎn)移酶(PCMT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Protein L-Isoaspartate-O-Methyltransferase (PCMT1) X16207 48T/96T Homo sapiens (Human)
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動力蛋白胞漿1重鏈1(DYNC1H1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1)X16210 48T/96T Homo sapiens (Human)
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