人非小細(xì)胞肺癌細(xì)胞;NCI-H23
形態(tài)特性 上皮樣
生長(zhǎng)特性 貼壁生長(zhǎng)
特征特性 人非小細(xì)胞肺癌細(xì)胞;NCI-H23該細(xì)胞源于一位51歲患有非小細(xì)胞肺癌黑人男性患者的治療前的腫瘤組織,表達(dá)C-myc、L-myc、v-src、v-abl、v-erb B、c-raf 1、Ha-ras、Ki-ras、N-ras RNAs;該細(xì)胞攜帶K-ras 12突變;p53基因246位密碼子突變ATC→ATG;表達(dá)PDGF A和B鏈的異源mRNA;表達(dá)TGFα、TGFβ和EGFR;角蛋白 5、8和18陽(yáng)性,波形蛋白陽(yáng)性,神經(jīng)絲蛋白陰性,左旋多巴脫氫酶陰性;據(jù)報(bào)道,在軟瓊脂中該細(xì)胞形成克隆的效率為9.7%。
培養(yǎng)條件 RPMI 1640 (w/o Hepes) 10%FBS;4.5g/L Glucose+10mMHepes+1mM丙酮酸鈉
傳代方法 1:3傳代;3~4天1次。
傳代情況
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
公司細(xì)胞廣泛用于國(guó)內(nèi)院校的細(xì)胞生物學(xué)研究,他們的很多論文都或多或少使用我們公司的細(xì)胞,作為實(shí)驗(yàn)項(xiàng)目研究
紫草酸 lithospermic acidX27547 98%
紫堇靈 Corynoline X27548 ≥98%
"紫杉葉素 " (+)-Taxifolin X27549 "≥98% "
"紫云英苷 " Astragalin X27550 "≥98% "
"祖師麻甲素(瑞香素) " Daphnetin X27551 "≥98% "
醉椒素 Kavain X27552 "≥99% "
左旋千金藤啶堿 L-Stepholidine X27553 "≥98% "
1,5-二咖啡酰奎寧酸 1,5-Dicaffeoylquinic acid X27554 ≥98%
1,8-二羥基葸醌 1,8-Dihydroxyanthraquinone X27555 ≥98.0%
10-姜酚 10-Gingerol X27556 ≥95%
10-羥基喜樹(shù)堿 10-Hydroxy camptothecin X27557 ≥95%
10-脫乙?;涂ǘ、?10-Deacetylbaccatine III X27558 ≥98%
11-氧-羅漢果皂甙V 11-oxo-mogroside V X27559 ≥98%
13-乙?;?9-羥基巴卡丁III 13-Acetyl-9-Dihydrobaccatin-III X27560 ≥98%
1-脫氧野尻霉素 1-Deoxynojirimycin X27561 ≥95%
2”-O-沒(méi)食子酰基金絲桃苷 2”-O-Galloylhyperin X27562 ≥98%
20(S)-人參皂苷 F1 20(S)-Ginsenoside F1(3b,6a,12b)-3,6,12-Trihydroxydammar-24-ene-20-yl beta-D-glucopyranoside X27563 ≥97%
20(S)-人參皂苷C-K 20(S)-Ginsenoside C-K X27564 ≥97%
20(S)-人參皂苷F2 20(S)-Ginsenoside-F2 X27565 ≥97%
20(S)-人參皂苷Rg3 Ginsenoside-Rg3 X27566 ≥97%
20(S)-人參皂苷-RH2 20(S)-Ginsenoside-RH2 X27567 ≥98%
23-羥基白樺酸 Anemosapogenin X27568 ≥98%
25-羥基-原人參二醇 25-OH-Protopanaxdiol X27569 ≥97%
25-羥基-原人參三醇 25-OH-Protopanaxtiol X27570 ≥97%
2-羥基澤蘭內(nèi)酯 2-hydroxyeupatolide X27571 ≥97%
3,6’-二芥子?;崽?3,6’-Disinapoyl sucrose X27572 ≥95%
3’-甲氧基葛根素 3’- Methoxy Puerarin X27573 ≥99%
3’-羥基葛根素 3'-hydroxy Puerarin X27574 ≥97%
4-去甲氧基鬼臼毒素 4'-Demethylpodophyllotoxin X27575 ≥97%
5-O-甲基維斯阿米醇苷 4'-O-beta-Glucopyranosyl-5-O-Methylvisamminol X27576 ≥98%
5-羥基色氨酸 5-HTP X27577 ≥98%
6,7-二甲氧基香豆素/濱蒿內(nèi)酯 Scoparone X27578 ≥98%
6-姜酚 6-Gingerol X27579 ≥95% ≥98%
6-姜烯酚 6-Shogaol X27580 94%
6-羥基-7甲氧基香豆素 6-hydroxy-7-methoxy-2H-chromen-2-one X27581 97%
7-乙基-10-羥基喜樹(shù)堿 7-Ethyl-10-hydroxycamptothecin X27582 ≥98%
7-乙基喜樹(shù)堿 7-Ethylcamptotrecin X27583 ≥95%
8-O-乙酰山梔苷甲酯 8-O-acetylshanzhiside methyl ester X27584 97%
8-甲氧基補(bǔ)骨脂素/花椒毒素 8-Methoxypsoralen 8-甲氧基補(bǔ)骨脂素 X27585 ≥98%
8-姜酚 8-Gingerol X27586 ≥95%
Rosiridin Rosiridin X27587 ≥95%
α-倒捻子素 Alpha-Mangostin X27588 ≥98%
α-細(xì)辛腦 Asarone X27589 ≥98%
α-香附酮 α-Cyperone X27590 ≥95%